Our results indicate that overexpression of the chimeric gene OsDST-SRDX improves salt tolerance of switchgrass, a C 4 biofuel crop.Ĭhimeric REpressor gene-silencing technology (CRES-T) DST SRDX domain Salt tolerance Switchgrass. Heres Why You Should Invest in Surmodics. eth domain names has escalated since amazon.eth received a purchase bid of 1m this month. The segment is a leader in developing an ELISA/EIA. This effect was verified by showing that DPBF4-SRDX fails to recognize the AK/HSD1 enhancer sequence in yeast one-hybrid assays, but increases heterodimmer. The transgenic plants had also higher activities of antioxidant enzymes associated with suppressed expressing of genes in H 2O 2 homeostasis, including glutathione S-transferase (GST2, GST6), cytochrome P450, peroxidase 24 precursor, and induced expressing of CAT and SOD under salt stress to eliminate excess H 2O 2. Surmodics (SRDX) is gaining strength from the In Vitro Diagnostics (IVD) unit. A dominant-negative version of DPBF4 fused to the SRDX repressor domain of SUPERMAN could counteract the repression and stimulate AK expression under low sugar and darkness in planta. They performed better under salt stress, had higher relative leaf water content, lower electrolyte leakage and lower malondialdehyde (MDA) content, and accumulated less Na + and more K + than WT controls. Compared to wild-type (WT) plants, OsDST-SRDX transgenic (TG) switchgrass plants showed wider leaves and thicker stems. To improve salt tolerance of switchgrass, a chimeric DROUGHT AND SALT TOLERANCE (DST) gene OsDST-SRDX was constructed using the Chimeric REpressor gene-Silencing Technology (CRES-T), and introduced into switchgrass genome by Agrobacterium-mediated transformation. Overexpression and functional suppression of OsNAC111 affect responses to M.
#Srdx domain Activator#
2003) repressed LUC reporter activity, confirming that fusion of the repressor domain converted OsNAC111 from an activator to a repressor (Fig. These observations suggest that the addition of the SRDX domain repressed the functions of OsPIL11 and OsPIL16 related to the maintenance of rice skotomorphogenesis. EAR-motif repression domain (SRDX) (Hiratsu et al. To avoid competing with crops in arable land utilization, improving salt tolerance of switchgrass is required to use marginal saline land for switchgrass production. However, OsPIL11-SRDX and OsPIL16-SRDX seedlings had typical photomorphogenic phenotypes, resembling WT seedlings grown under light (Fig. The ectopic expression of SRDXWUSm1 was effective in inducing adventitious shoot formation but not somatic embryogenesis (Figure 2E). Switchgrass (Panicum virgatum L.) is a forage and model feedstock plant. However, the position of the SRDX repressor domain is important because its addition to the C-terminus was not effective (Figure 2E). Overexpression of OsDST-SRDX chimeric gene in switchgrass promotes plant growth and improves the salt tolerance of transgenic switchgrass by improving its antioxidative ability.
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